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PNAS:拟南芥CSLC基因参与木葡聚糖生物合成(2)
作者:网站采编关键词:
摘要:: Kenneth Keegstra Abstract 背景回顾 : Xyloglucan ( XyG )is an abundant component of the primary cell walls of most plants. 提出问题 :While the structure of XyG has been well studied, much rem
Abstract
背景回顾:Xyloglucan(XyG)is an abundant component of the primary cell walls of most plants.
提出问题:While the structure of XyG has been well studied, much remains to be learned about itsbiosynthesis.
主要研究:Here we employed reverse genetics to investigate the role ofArabidopsiscellulose synthase like-C(CSLC)proteins inXyG biosynthesis.
结果-单突:We found that single mutants containingaT-DNA in each of the fiveArabidopsis CSLCgenes hadnormal levels of XyG.
结果-多突:However, higher-ordercslcmutants had significantly reduced XyG levels, andamutant with disruptions in all fiveCSLCgenes had no detectable XyG.The higher-order mutants grew with mild tissue-specific phenotypes. Despite the apparent lack of XyG, thecslcquintuple mutant did not display significant alteration of gene expression at the whole-genome level, excluding transcriptional compensation. The quintuple mutant could be complemented by each of the fiveCSLCgenes, supporting the conclusion that each of them encodes a XyG glucan synthase.
结果-进化:Phylogenetic analyses indicated that theCSLCgenes are widespread in the plant kingdom and evolved from an ancient family.
结论:These results establish the role of theCSLCgenes in XyG biosynthesis, and the mutants described here provide valuable tools with which to study both the molecular details of XyG biosynthesis and the role of XyG in plant cell wall structure and function.
摘 要
木葡聚糖在大多数的植物中均是初生细胞壁的富含成分。虽然XyG的结构已经研究得很深入了,但是其生物合成方面的研究尚有欠缺。本文中,作者通过反向遗传学的方法研究了拟南芥类纤维素合酶CSLC蛋白在XyG生物合成中的作用。作者发现T-DNA插入的所有CSLC基因单突植株均具有正常的XyG含量。然而,高阶
文章来源:《合成纤维》 网址: http://www.hcqwzz.cn/zonghexinwen/2020/1106/339.html